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mini opticon real time pcr system  (Bio-Rad)


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    Structured Review

    Bio-Rad mini opticon real time pcr system
    Mini Opticon Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 8213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+opticon+real+time+pcr+system/MiniOpticon+Real-Time+PCR+Detection+System/pmc12779942-89-9-13
    Average 96 stars, based on 8213 article reviews
    mini opticon real time pcr system - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: High light intensity enhances cannabinoid biosynthesis through concerted gene expression in hemp ( Cannabis sativa ) flowers.
    Article Snippet: First-strand cDNA synthesis for qRT-PCR was accomplished using the ReverTra Ace-a-kit (Toyobo, Osaka, Japan) with oligo (dT) 20 primers from the extracted total RNA. .. Expression analysis of the seven cannabinoid biosynthesis-associated genes was conducted by quantitative real-time PCR using a Mini Opticon Real-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA). .. The thermal cycling protocol included: initial denaturation at 95°C for 3 min; followed by 35 amplification cycles consisting of 95°C for 15 s, 54°C for 20 s, and 72°C for 15 s; with final steps of 95°C for 10 s and 65°C for 5 s. PCR reactions were performed in 20 mL volumes containing 0.4 mMprimer concentrations and 1× SYBR Green RealTime PCR master mix (Toyobo, Osaka, Japan).

    Article Title: High light intensity enhances cannabinoid biosynthesis through concerted gene expression in hemp ( Cannabis sativa ) flowers
    Article Snippet: First-strand cDNA synthesis for qRT-PCR was accomplished using the ReverTra Ace-α-kit (Toyobo, Osaka, Japan) with oligo (dT) 20 primers from the extracted total RNA. .. Expression analysis of the seven cannabinoid biosynthesis-associated genes was conducted by quantitative real-time PCR using a Mini Opticon Real-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA). .. The thermal cycling protocol included: initial denaturation at 95°C for 3 min; followed by 35 amplification cycles consisting of 95°C for 15 s, 54°C for 20 s, and 72°C for 15 s; with final steps of 95°C for 10 s and 65°C for 5 s. PCR reactions were performed in 20 μL volumes containing 0.4 μM primer concentrations and 1× SYBR Green Real-Time PCR master mix (Toyobo, Osaka, Japan).

    Real-time Polymerase Chain Reaction:

    Article Title: High light intensity enhances cannabinoid biosynthesis through concerted gene expression in hemp ( Cannabis sativa ) flowers.
    Article Snippet: First-strand cDNA synthesis for qRT-PCR was accomplished using the ReverTra Ace-a-kit (Toyobo, Osaka, Japan) with oligo (dT) 20 primers from the extracted total RNA. .. Expression analysis of the seven cannabinoid biosynthesis-associated genes was conducted by quantitative real-time PCR using a Mini Opticon Real-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA). .. The thermal cycling protocol included: initial denaturation at 95°C for 3 min; followed by 35 amplification cycles consisting of 95°C for 15 s, 54°C for 20 s, and 72°C for 15 s; with final steps of 95°C for 10 s and 65°C for 5 s. PCR reactions were performed in 20 mL volumes containing 0.4 mMprimer concentrations and 1× SYBR Green RealTime PCR master mix (Toyobo, Osaka, Japan).

    Article Title: Engineered ATP-Loaded Extracellular Vesicles Derived from Mesenchymal Stromal Cells: A Novel Strategy to Counteract Cell ATP Depletion in an In Vitro Model
    Article Snippet: Total RNA was then quantified by a NanoDropTM (Thermo-Fisher Scientific). cDNAs obtained from 500 ng of RNA were reverse transcribed using an iScriptTM cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) and quantitative PCR analysis was performed using the oligonucleotide primers displayed in . .. The reaction was carried out by using a mini-opticon Real-Time PCR system (BioRad, Hercules, CA, USA) and data analysis was performed by CFX Manager software v1.5.534.0511. ..

    Article Title: Butyrate prevents chemotherapy-induced gastrointestinal toxicity and microbial dysbiosis
    Article Snippet: .. Reverse-transcriptase polymerase chain reaction (RT-PCR) was performed on a Mini-Opticon real-time PCR system (Bio-Rad, Hercules, CA). ..

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells.
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (BioRad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Article Title: Transcriptomics profiling and WGCNA provide new insights into anthocyanin metabolism in spring orchid (Cymbidium goeringii) flowers for future industrial applications
    Article Snippet: The qRT-PCR assays was performed using the SYBR Premix Ex Taq kit (Takara, Japan), following the manufacturer’s instructions. .. Three independent biological replicates for per gene. and Amplification reactions were conducted on the Mini Opticon Real-time PCR System (Bio-Rad, USA). .. Statistical analyses were conducted using the SPSS software version 19.0 (IBM Corporation, Armonk, NY, USA).

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (Bio-Rad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Article Title: High light intensity enhances cannabinoid biosynthesis through concerted gene expression in hemp ( Cannabis sativa ) flowers
    Article Snippet: First-strand cDNA synthesis for qRT-PCR was accomplished using the ReverTra Ace-α-kit (Toyobo, Osaka, Japan) with oligo (dT) 20 primers from the extracted total RNA. .. Expression analysis of the seven cannabinoid biosynthesis-associated genes was conducted by quantitative real-time PCR using a Mini Opticon Real-time PCR system (Bio-Rad Laboratories, Hercules, CA, USA). .. The thermal cycling protocol included: initial denaturation at 95°C for 3 min; followed by 35 amplification cycles consisting of 95°C for 15 s, 54°C for 20 s, and 72°C for 15 s; with final steps of 95°C for 10 s and 65°C for 5 s. PCR reactions were performed in 20 μL volumes containing 0.4 μM primer concentrations and 1× SYBR Green Real-Time PCR master mix (Toyobo, Osaka, Japan).

    Article Title: Asiaticoside-Loaded Nanosponges Hydrogel Has an Anti-inflammatory Effect and Promotes Human Dental Pulp Regeneration.
    Article Snippet: Introduction: Asiaticoside (AS) demonstrated potential in wound healing and antiinflammation.. However, its therapeutic applications are limited due to poor solubility and low bioavailability, which make it difficult to use as dental pulp capping agent.. The nanosponges (Ns) are nanosized carriers capable of carrying small drug molecules.

    Software:

    Article Title: Engineered ATP-Loaded Extracellular Vesicles Derived from Mesenchymal Stromal Cells: A Novel Strategy to Counteract Cell ATP Depletion in an In Vitro Model
    Article Snippet: Total RNA was then quantified by a NanoDropTM (Thermo-Fisher Scientific). cDNAs obtained from 500 ng of RNA were reverse transcribed using an iScriptTM cDNA synthesis kit (Bio-Rad, Hercules, CA, USA) and quantitative PCR analysis was performed using the oligonucleotide primers displayed in . .. The reaction was carried out by using a mini-opticon Real-Time PCR system (BioRad, Hercules, CA, USA) and data analysis was performed by CFX Manager software v1.5.534.0511. ..

    Reverse Transcription:

    Article Title: Butyrate prevents chemotherapy-induced gastrointestinal toxicity and microbial dysbiosis
    Article Snippet: .. Reverse-transcriptase polymerase chain reaction (RT-PCR) was performed on a Mini-Opticon real-time PCR system (Bio-Rad, Hercules, CA). ..

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells.
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (BioRad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (Bio-Rad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Polymerase Chain Reaction:

    Article Title: Butyrate prevents chemotherapy-induced gastrointestinal toxicity and microbial dysbiosis
    Article Snippet: .. Reverse-transcriptase polymerase chain reaction (RT-PCR) was performed on a Mini-Opticon real-time PCR system (Bio-Rad, Hercules, CA). ..

    SYBR Green Assay:

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells.
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (BioRad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (Bio-Rad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Control:

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells.
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (BioRad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Article Title: HBx/WDR5 enhances IGF-1 transcription in hepatocellular carcinoma cells and promotes recruitment, infiltration, and activity of Treg cells
    Article Snippet: The RNA isolation kit (Promega) was used to separate total RNA from cells. .. Reverse transcription was conducted using a Reverse Transcription Kit (TOYOBO). qPCR was carried out utilizing the Mini Opticon Real-time PCR system (Bio-Rad) and SYBR Green (Invitrogen, USA). β-actin was used as an internal control. ..

    Amplification:

    Article Title: Transcriptomics profiling and WGCNA provide new insights into anthocyanin metabolism in spring orchid (Cymbidium goeringii) flowers for future industrial applications
    Article Snippet: The qRT-PCR assays was performed using the SYBR Premix Ex Taq kit (Takara, Japan), following the manufacturer’s instructions. .. Three independent biological replicates for per gene. and Amplification reactions were conducted on the Mini Opticon Real-time PCR System (Bio-Rad, USA). .. Statistical analyses were conducted using the SPSS software version 19.0 (IBM Corporation, Armonk, NY, USA).



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